Once you have created a gate (except when you use the. To draw other types of gates, select the appropriate tool. Note that you can hold down the option key while clicking, to toggle between the rectangle and polygon gating tool. If your preference is to have FlowJo automatically select a gating tool, you can choose to have the polygons or rectangles tool as your default for bivariate displays.
Boolean gating in flowjo 10 download#
Obviously, if you don't have FlowJo yet, then download it from the website. Thus, here's a short set of instructions for using it to do a basic process, such as determining what percentage of live cells are also GFP positive. While I've been using FlowJo for a long time, I realize it isn't super intuitive and new people to the lab may first struggle in using it. Helps correct for technical variability within FlowJo by normalizing batches of flow data. The Workspace contains a list of all of the data samples that you load, the gates, statistics and other analyses that you apply, and the table and graphical layout that you design Automatically Find the Shortest Gating Strategy to a Population of Interest. This is called a Gating Tree FlowJo is an integrated environment for viewing and analysing flow cytometric data, presented in the form of a Workspace. When you create gates in a sample or subpopulation, FlowJo shows the new subsets in the Workspace in a hierarchical manner each subpopulation is shown indented under the sample or subset that it came from.
The backgating icon shows up graph window for a subset that is gated more than once (or you can choose Backgating Analysis. FlowJo's backgating analysis provides a tool to view the effect of every gate in the gating tree on the final gated population. 2) Take (gate) 3 and apply it to (gate) 1, creating a new subpopulation we'll call 3b. You tell FlowJo to backgatehere is what FlowJo does: 1) Take (gate) 3 and apply it to (gate) 2 (you'll see no change in the workspace). Isolate, divide, or group your populations for excellent annotation and analysis